The NF-kB Inhibitory Proteins IkBa and IkBb Mediate Disparate Responses to Inflammation in Fetal Pulmonary Endothelial Cells

The effects of labour and of interleukin 1 beta upon the expression of nuclear factor kappa B related proteins in human amnion.

Human labour is associated with persistently increased nuclear factor kappa B (NF-kB) activity in amnion. In this study we have shown that this involves only the p65 and p50 NF-kB subunits and is associated with an increase in the expression of p65 (P < 0.05), and of the NF-kB binding proteins IkBa, IkBb-1 and IkBb-2 (P < 0.05). Interleukin-1b stimulation leads to rapid degradation and resynthe...

The NF-κB inhibitory proteins IκBα and IκBβ mediate disparate responses to inflammation in fetal pulmonary endothelial cells.

Exposure to intrauterine inflammation impairs lung growth but paradoxically protects the neonatal pulmonary vasculature from hyperoxic injury. The mechanisms mediating these contradictory effects are unknown. The objective is to identify the role of NF-κB in mediating cytoprotective and proinflammatory responses to inflammation in the fetal pulmonary endothelium. In newborn rats exposed to intr...

Nuclear factor-kB activation by the photochemotherapeutic agent verteporfin

The nuclear factor-kappa B (NF-kB) gene transactivator serves in the formation of immune, inflammatory, and stress responses. In quiescent cells, NF-kB principally resides within the cytoplasm in association with inhibitory k (IkB) proteins. The status of IkB and NF-kB proteins was evaluated for promyelocytic leukemia HL-60 cells treated at different intensities of photodynamic therapy (PDT). T...

Listeria monocytogenes infection of P388D1 macrophages results in a biphasic NF-kB (RelAyp50) activation induced by lipoteichoic acid and bacterial phospholipases and mediated by IkBa and IkBb degradation

As previously reported, Listeria monocytogenes infection of P388D1 macrophages results in a rapid induction of NF-kB DNA-binding activity. Here we show that this induction of NF-kB activity occurs in a biphasic mode: first, a transient, IkBa degradation-dependent phase of activity, also induced by the nonvirulent species Listeria innocua, which is mediated by binding of the bacteria to the macr...

Synergistic Activation of NF-kB by Tumor Necrosis Factor Alpha and Gamma Interferon via Enhanced IkBa Degradation and De Novo IkBb Degradation